Journal: Frontiers in Veterinary Science

Article Title: Detection of bimodal survivin expressions in canine cancer types by flow cytometry compared to immunohistochemistry

doi: 10.3389/fvets.2025.1552415

Figure Lengend Snippet: Analysis of survivin expression sites by IHC and WB. (A) Images of HE staining and survivin expression via IHC in canine melanoma cell lines (CMM2, CMeC, and LMeC) are shown. (B) Images of HE staining and survivin expression via IHC in murine cell lines (p815, CT26, and B16F10) are shown. (C) The cytosol and total survivin expression patterns via WB are indicated. (D) The expression levels of total survivin (blue bar) and cytosolic survivin (orange bar) corrected on the basis of β-actin expression are shown via density of plot analysis via ImageJ software.

Article Snippet: A total of six cell lines were used: canine malignant melanoma lines [CMM2, CMeC2, LMeC; provided by Dr. Takayuki Nakagawa, Department of Veterinary Surgery, University of Tokyo; ( )], the murine malignant melanoma line B16F10, the murine mast cell tumor line p815, and the murine colon cancer line CT26 (distributed for a fee by the JCRB Cell Bank).

Techniques: Expressing, Staining, Software

Journal:

Article Title: Vascular endothelial growth factor overproduced by tumour cells acts predominantly as a potent angiogenic factor contributing to malignant progression

doi: 10.1046/j.1365-2613.1999.00122.x

Figure Lengend Snippet: (a) VEGF and (b) bFGF productivity of cancer cells transfected with VEGF cDNA. ▪ parental cells; □ transfected clones. The levels of VEGF and bFGF contained in conditioned medium and cell extract, respectively, were determined by sandwich ELISA. Sample values were plotted against a recombinant VEGF or bFGF standard curve (mean ± SE, n = 3). ND: not detectable. QG90, RPMI4788, MCF-7 and their transfected clones were cultured in RPMI1640 containing 10% FCS (normal). Hormones-stripped FCS with (E2+) or without (E2−) 10−8 M 17 β-oestradiol was used for MCF-7 and its transfected clone, M2–24-G10.

Article Snippet: The human colon cancer cell line, RPMI4788 ( Moore & Koike 1964 ; Naomoto et al . 1987 ), was a generous gift from Dr Akio Hizuta (First Department of Surgery, Okayama University Medical School, Okayama, Japan).

Techniques: Transfection, Clone Assay, Sandwich ELISA, Recombinant, Cell Culture

Journal:

Article Title: Vascular endothelial growth factor overproduced by tumour cells acts predominantly as a potent angiogenic factor contributing to malignant progression

doi: 10.1046/j.1365-2613.1999.00122.x

Figure Lengend Snippet: HUVE cell growth stimulatory activities in conditioned media of VEGF-transfected clones of (a) ○ QG90; □ Q1-1-C1; • Q1-24-E6; ▪ Q1-24-F6; ▴ Q1-24-H2, (b) ○ RPMI4788; • S0A12; ▪ S2G2 and (c) ○ MCF-7; • M2-24-D10; ▪ M2-24-E11; □ M2-24-G10 cells. HUVE cells were cultured in the presence of various concentrations of conditioned medium from each clone, indicated in Figure 1. After 4 days, HUVE cell growth was determined by MTT assay. The HUVE cell growth induced by 2.5 ng/ml of human recombinant VEGF165 was used as a positive control (♦). Results are presented as the percentage of the control without any growth factor. Each point indicates the mean of duplicate determinations.

Article Snippet: The human colon cancer cell line, RPMI4788 ( Moore & Koike 1964 ; Naomoto et al . 1987 ), was a generous gift from Dr Akio Hizuta (First Department of Surgery, Okayama University Medical School, Okayama, Japan).

Techniques: Transfection, Clone Assay, Cell Culture, MTT Assay, Recombinant, Positive Control, Control

Journal:

Article Title: Vascular endothelial growth factor overproduced by tumour cells acts predominantly as a potent angiogenic factor contributing to malignant progression

doi: 10.1046/j.1365-2613.1999.00122.x

Figure Lengend Snippet: Growth curves of VEGF-transfected clones of (a) ○ QG90; □ Q1-1-C1; • Q1-24-E6; ▪ Q1-24-F6; ▴ Q1-24-H2, (b) ○ RPMI4788; • S0A12; ▪ S2G2 and (c) ○ MCF-7; • M2-24-D10; ▪ M2-24-E11; □ M2-24-G10 cells. The number of cells was counted with a Coulter Counter (mean ± SE, n = 3).

Article Snippet: The human colon cancer cell line, RPMI4788 ( Moore & Koike 1964 ; Naomoto et al . 1987 ), was a generous gift from Dr Akio Hizuta (First Department of Surgery, Okayama University Medical School, Okayama, Japan).

Techniques: Transfection, Clone Assay

Journal:

Article Title: Vascular endothelial growth factor overproduced by tumour cells acts predominantly as a potent angiogenic factor contributing to malignant progression

doi: 10.1046/j.1365-2613.1999.00122.x

Figure Lengend Snippet: Tumorigenic potentials of transfected clones of QG90, RPMI4788 and MCF-7 cells

Article Snippet: The human colon cancer cell line, RPMI4788 ( Moore & Koike 1964 ; Naomoto et al . 1987 ), was a generous gift from Dr Akio Hizuta (First Department of Surgery, Okayama University Medical School, Okayama, Japan).

Techniques: Transfection, Clone Assay

Journal:

Article Title: Vascular endothelial growth factor overproduced by tumour cells acts predominantly as a potent angiogenic factor contributing to malignant progression

doi: 10.1046/j.1365-2613.1999.00122.x

Figure Lengend Snippet: In vivo angiogenic activity of VEGF-transfected clones of (a) QG90 and (b) RPMI4788 cells. A Millipore chamber containing each clone or HBSS was implanted s.c. into a dorsal air sac of a BALB/c mouse (three to six mice/group). After 4 days, the area of new capillarization induced by each clone was determined with an image analyser. Results (mean ± SE) are presented as percentages of the mean area of the control group treated with a HBSS-containing chamber. * P < 0.01 vs. the control group; ns, not significant (by Student's t-test).

Article Snippet: The human colon cancer cell line, RPMI4788 ( Moore & Koike 1964 ; Naomoto et al . 1987 ), was a generous gift from Dr Akio Hizuta (First Department of Surgery, Okayama University Medical School, Okayama, Japan).

Techniques: In Vivo, Activity Assay, Transfection, Clone Assay, Control

Journal:

Article Title: Vascular endothelial growth factor overproduced by tumour cells acts predominantly as a potent angiogenic factor contributing to malignant progression

doi: 10.1046/j.1365-2613.1999.00122.x

Figure Lengend Snippet: Hematoxylin and eosin-stained sections of tumours 32 days after s.c. injection of (a) RPMI4788 cells and the transfected clones, (b) S0A12 and (c) S2G2 cells into nude mice. RPMI4788 cells yielded small, poorly vascularlized tumours (a), whereas both clones yielded well-vascularlized ones (b, c). Arrow heads indicate blood vessels. Scale bar = 400μm.

Article Snippet: The human colon cancer cell line, RPMI4788 ( Moore & Koike 1964 ; Naomoto et al . 1987 ), was a generous gift from Dr Akio Hizuta (First Department of Surgery, Okayama University Medical School, Okayama, Japan).

Techniques: Staining, Injection, Transfection, Clone Assay